File:Transportation-of-Nanoscale-Cargoes-by-Myosin-Propelled-Actin-Filaments-pone.0055931.s011.ogv
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Transportation-of-Nanoscale-Cargoes-by-Myosin-Propelled-Actin-Filaments-pone.0055931.s011.ogv (Ogg Theora video file, length 23 s, 256 × 256 pixels, 286 kbps, file size: 788 KB)
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DescriptionTransportation-of-Nanoscale-Cargoes-by-Myosin-Propelled-Actin-Filaments-pone.0055931.s011.ogv |
English: HMM-propelled actin filaments with streptavidin coated quantum dots attached to the filaments via biotinylated actin. The actin filaments were also labelled with Alexa-488 phalloidin. The movie first illustrates a few frames with the FITC filter set to observe the entire filaments whereas the remaining frames were obtained using the TRITC filter set to observe the quantum dots with emission maximum at 605 nm. Filaments and quantum dots pseudocolored to indicate their different emission maxima. Note, 1. that only a small fraction of the filaments are labelled with quantum dots and 2. that there is a substantial number of non-specifically bound quantum dots, falsely indicating a large number of stationary actin filaments with quantum dots. From series of experiments in Fig. 2 (a80 solution). Frame rate: 5 s−1 (real time) and image size: 85×85 µm2. |
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Source | Movie S3 from Persson M, Gullberg M, Tolf C, Lindberg A, Mansson A, Kocer A (2013). "Transportation of Nanoscale Cargoes by Myosin Propelled Actin Filaments". PLOS ONE. DOI:10.1371/journal.pone.0055931. PMID 23437074. | ||
Author | Persson M, Gullberg M, Tolf C, Lindberg A, Mansson A, Kocer A | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:41, 4 March 2013 | 23 s, 256 × 256 (788 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Persson M, Gullberg M, Tolf C, Lindberg A, Mansson A, Kocer A |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |