File:Trapping-of-normal-EB1-ligands-in-aggresomes-formed-by-an-EB1-deletion-mutant-1471-2121-6-17-S5.ogv
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DescriptionTrapping-of-normal-EB1-ligands-in-aggresomes-formed-by-an-EB1-deletion-mutant-1471-2121-6-17-S5.ogv |
English: Additional File 5 Perinuclear EB1-ΔN2-GFP aggregate assembly is microtubule-dependent. Image capture rate was 1 frame/10s using a 63X oil immersion lens, playback rate is 20 frames/s. Cells were incubated in nocodazole for 4 h followed by a wash and the addition of fresh imaging medium without drug. Imaging was initiated 30 min after nocodazole wash out. In the absence of drug EB1-ΔN2-GFP aggregates regain motility. Movement is intermittent and with an overall retrograde bias leading to the growth of perinuclear aggregates. |
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Source | Riess N, Milward K, Lee T, Adams M, Askham J, Morrison E (2005). "Trapping of normal EB1 ligands in aggresomes formed by an EB1 deletion mutant". BMC Cell Biology. DOI:10.1186/1471-2121-6-17. PMID 15813965. PMC: 1090566. | ||
Author | Riess N, Milward K, Lee T, Adams M, Askham J, Morrison E | ||
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current | 01:35, 21 November 2012 | 4.0 s, 256 × 255 (949 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional File 5 |
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Author | Riess N, Milward K, Lee T, Adams M, Askham J, Morrison E |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Perinuclear EB1-?N2-GFP aggregate assembly is microtubule-dependent. Image capture rate was 1 frame/10s using a 63X oil immersion lens, playback rate is 20 frames/s. Cells were incubated in nocodazole for 4 h followed by a wash and the addition of fresh imaging medium without drug. Imaging was initiated 30 min after nocodazole wash out. In the absence of drug EB1-?N2-GFP aggregates regain motility. Movement is intermittent and with an overall retrograde bias leading to the growth of perinuclear aggregates. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2005 |