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File:Unprecedented-Cell-Selection-Using-Ultra-Quick-Freezing-Combined-with-Aquaporin-Expression-pone.0087644.s004.ogv

Unprecedented-Cell-Selection-Using-Ultra-Quick-Freezing-Combined-with-Aquaporin-Expression-pone.0087644.s004.ogv ‎(Ogg Theora video file, length 5.6 s, 200 × 200 pixels, 236 kbps, file size: 160 KB)

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English: Time-lapse imaging of ET1-induced intracellular calcium with 3 cycles of ultra-quick freezing/thawing. CHO cells were transiently transfected with ETAR-IRES-EGFP. It was assessed after 3 cycles of ultra-quick freezing/thawing (see Figure 4D, top). Intracellular calcium imaging in response to endothelin-1 (ET1). The cells were pre-loaded with the calcium indicator Oregon Green/AM (see Materials and Methods). Calcium imaging is shown using a video obtained after 3rd ultra-quick freezing (Video S1). Bar, 20 µm. Frame size is 320×320 µm.
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Source Video S1 from Kato Y, Miyauchi T, Abe Y, Kojic D, Tanaka M, Chikazawa N, Nakatake Y, Ko S, Kobayashi D, Hazama A, Fujiwara S, Uchida T, Yasui M (2014). "Unprecedented Cell-Selection Using Ultra-Quick Freezing Combined with Aquaporin Expression". PLOS ONE. DOI:10.1371/journal.pone.0087644. PMID 24558371. PMC: 3928110.
Author Kato Y, Miyauchi T, Abe Y, Kojic D, Tanaka M, Chikazawa N, Nakatake Y, Ko S, Kobayashi D, Hazama A, Fujiwara S, Uchida T, Yasui M
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current04:44, 25 February 20145.6 s, 200 × 200 (160 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 71 kbps Completed 10:09, 21 December 2023 1.0 s
WebM 360P 99 kbps Completed 20:21, 2 December 2023 1.0 s
Streaming 144p (MJPEG) 303 kbps Completed 21:26, 11 November 2023 1.0 s

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