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File:Use-of-KikGR-a-photoconvertible-green-to-red-fluorescent-protein-for-cell-labeling-and-lineage-1471-213X-9-49-S1.ogv

Use-of-KikGR-a-photoconvertible-green-to-red-fluorescent-protein-for-cell-labeling-and-lineage-1471-213X-9-49-S1.ogv ‎(Ogg Theora video file, length 19 s, 519 × 512 pixels, 475 kbps, file size: 1.1 MB)

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English: Live imaging and photoconversion of KikGR, a green-to-red fluorescent protein, in a mouse embryo. 3D time-lapse of an E7.5 (early headfold stage - EHF) CAG::KikGR embryo from the EHF - ESom (early somite stage). KikGR is a green-to-red photoconvertible fluorescent protein. In the CAG::KikGR strain all cells express KikGR. There are three consecutive sequences in the movie. The first depicts the bright field channel alone to illustrate normal development (foregut invagination, development of the headfolds as well as somite condensation), second the red fluorescent channel (post conversion) overlayed on bright field, and third the red fluorescent channel. Two square regions of interest (ROIs) have been converted within the mesoderm. Each frame depicting fluorescence represents a 3D reconstruction of a z-stack (266 μm). The interval between slices is 7 μm. The time interval between z-stacks is 20 min. The time-lapse sequence covers 9 hours and 20 min. The embryo is suspended from its ectoplacental cone using a human eyelash. Anterior of the embryo is to the top left.
Date
Source Nowotschin S, Hadjantonakis A (2009). "Use of KikGR a photoconvertible green-to-red fluorescent protein for cell labeling and lineage analysis in ES cells and mouse embryos". BMC Developmental Biology. DOI:10.1186/1471-213X-9-49. PMID 19740427. PMC: 2872819.
Author Nowotschin S, Hadjantonakis A
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current00:18, 21 November 201219 s, 519 × 512 (1.1 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 124 kbps Completed 22:57, 24 October 2018 7.0 s
Streaming 480p (VP9) Not ready Unknown status
VP9 360P 67 kbps Completed 22:56, 24 October 2018 5.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 33 kbps Completed 22:56, 24 October 2018 3.0 s
Streaming 240p (VP9) 33 kbps Completed 11:14, 21 December 2023 1.0 s
WebM 360P 485 kbps Completed 01:28, 21 November 2012 7.0 s
Streaming 144p (MJPEG) 111 kbps Completed 02:07, 12 November 2023 1.0 s

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