English: Tomogram and model of a PyV-infected nucleus. A movie compiled from images obtained as a 2×2 montage over six serial thick sections (300 nm) with each section imaged as a tilt-series (±60°) of a PyV-infected 3T3 nucleus (1.8 µm total thickness). The movie progresses through the tilt-series of each section, followed by modeling of each section and concludes with a 3-D representation of the infected nucleus. Purple = condensed chromatin or chromatin-like patches; red = virions; yellow = tubular structures. Although in each section the virus factories appear separate from one other, when the sections are combined the tubular structures (yellow cylinders) appear to connect each of the virus clusters (red spheres). Single images were extracted from the movie and are shown in Figure 1B (tomogram) and 1D (3-D model). (MP4)