English: Dynamic simulation of an experimental mixed haploid population. In the movie generated from confocal images to the left, images are overlays of the bright field channel, GFP channel (MATa Fus1-GFP) and mCherry channel (MATα mCherry). The simulated α-factor distribution is shown on the right. In the simulation red indicates MATα cells and white MATa cells. Each second in the movie corresponds to 5 minutes in real time. Due to the delay in the pheromone dependent induction of Bar1, α-factor will initially accumulate to high concentrations, which is counteracted by a strong degradation of α-factor caused by Bar1 induction after 30 minutes. Finally, the α-factor level rises again due to the loss of Bar1 induction to its steady state value. It should be noted that shmoo formation seems to take place shortly after the final stable gradient has been formed, indicating a good timing between regulation of the extracellular α-factor distribution and mating. The movie requires the Xvid codec (http://www.xvid.org).