File:Zebra fish U0126 application during a specific time-window decreases ERK phosphorylation specifically.jpeg
Size of this preview: 343 × 599 pixels. Other resolutions: 137 × 240 pixels | 275 × 480 pixels | 440 × 768 pixels | 586 × 1,024 pixels | 1,826 × 3,189 pixels.
Original file (1,826 × 3,189 pixels, file size: 1.3 MB, MIME type: image/jpeg)
File information
Structured data
Captions
Summary edit
DescriptionZebra fish U0126 application during a specific time-window decreases ERK phosphorylation specifically.jpeg | Zebra fish. U0126 application during a specific time-window decreases ERK phosphorylation specifically. A: Diphospho-Erk staining in the trunk of wt 24 hpf embryos.B: U0126 eliminates ERK1/2 phosphorylation (green) in the trunk and tail of 24 hpf zebrafish embryos. Notice that U0126-treated embryos have been overexposed (can be judged by the autofluorescence of the yolk sac extension) and still fail to show specific staining. C: U0124 does not eliminate the p-Erk staining. D: PD98059 also does not eliminate p-Erk staining when given just below the level of lethal toxicity (25 μM). E: Somewhat weaker but still significant p-Erk staining in embryos treated with U0126 starting at 10 hpf. F: Western-blot analysis demonstrated a slight but incomplete reduction in pErk1/2 levels in 10-somite embryos.G: Stage and dose-dependency of U0126 application. There is a dramatic drop in the percentage of affected embryos when treatment is applied after epiboly is completed. H: Washout experiments reveal the necessity of U0126 to be present until at least the 18 somite stage to produce the full phenotype. |
Date | |
Source | https://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-8-42#Fig1 https://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-8-42 Hawkins, T.A., Cavodeassi, F., Erdélyi, F. et al. The small molecule Mek1/2 inhibitor U0126 disrupts the chordamesoderm to notochord transition in zebrafish. BMC Dev Biol 8, 42 (2008). |
Author | Hawkins, T.A., Cavodeassi, F., Erdélyi, F. et al. |
This file, which was originally posted to an external website, has not yet been reviewed by an administrator or reviewer to confirm that the above license is valid. See Category:License review needed for further instructions.
|
Rights and permissions
Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Licensing edit
This file is licensed under the Creative Commons Attribution 2.0 Generic license.
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 00:29, 25 April 2024 | 1,826 × 3,189 (1.3 MB) | Rasbak (talk | contribs) | {{Information |description=Zebra fish. U0126 application during a specific time-window decreases ERK phosphorylation specifically. A: Diphospho-Erk staining in the trunk of wt 24 hpf embryos.B: U0126 eliminates ERK1/2 phosphorylation (green) in the trunk and tail of 24 hpf zebrafish embryos. Notice that U0126-treated embryos have been overexposed (can be judged by the autofluorescence of the yolk sac extension) and still fail to show specific staining. C: U0124 does not eliminate the p-Erk st... |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.