File:Перехід барвника Hoechst 33342.jpg

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English: Bull sperm, which was painted by fluorescent DNA-dye Hoechst 33342, were three times washed (ie centrifugation, resuspended in medium without dye Hoechst 33342) and then added unpainted cells (pictured left, unpainted cells are dark, not illuminated). After 1.5 hours incubation of the mix, all cells began to fluoresce, there was redistribution of Hoechst 33342 dye (photo right). Mammalian spermatozoa have membrane pumps (ABC efflux pump), which can to pump the dye into outside. Whether is it an active process, or passive diffusion? Experiments continue.
English: Hoechst 33342 redistribution
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Author BioRes Oleg

Department of Biotechnology, Institute of Animal Husbandry, Kharkov, Ukraine

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Date/TimeThumbnailDimensionsUserComment
current21:44, 31 October 2015Thumbnail for version as of 21:44, 31 October 20151,538 × 512 (204 KB)BioRes Oleg (talk | contribs)User created page with UploadWizard

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