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File:Activation-of-RhoA-but-Not-Rac1-Mediates-Early-Stages-of-S1P-Induced-Endothelial-Barrier-Enhancement-pone.0155490.s003.ogv

Activation-of-RhoA-but-Not-Rac1-Mediates-Early-Stages-of-S1P-Induced-Endothelial-Barrier-Enhancement-pone.0155490.s003.ogv ‎(Ogg Theora video file, length 7.5 s, 234 × 296 pixels, 290 kbps, file size: 266 KB)

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English: S1P activated RhoA primarily at cell periphery. This movie shows time lapse images of an endothelial cell expressing the pTriEx-RhoA FLARE.sc Biosensor used to detect RhoA in its GTP-bound, active form. The top panel shows the CFP channel, the middle panel shows the YFP channel, and the bottom panel portrays the YFP:CFP ratio (FRET) indicating RhoA activation. The frames are at 1 min intervals. S1P (2 μM) was added at time = 0 min. The addition caused an obvious shift in GTP-bound RhoA toward the cell periphery in the first minute after the addition of S1P.
Date
Source S1 Movie from Zhang X, Adderley S, Breslin J (2016). "Activation of RhoA, but Not Rac1, Mediates Early Stages of S1P-Induced Endothelial Barrier Enhancement". PLOS ONE. DOI:10.1371/journal.pone.0155490. PMID 27187066. PMC: 4871357.
Author Zhang X, Adderley S, Breslin J
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current04:02, 13 October 20167.5 s, 234 × 296 (266 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 86 kbps Completed 17:45, 16 August 2018 2.0 s
Streaming 240p (VP9) 86 kbps Completed 14:59, 9 December 2023 1.0 s
WebM 360P 157 kbps Completed 15:23, 16 November 2023 0.0 s
Streaming 144p (MJPEG) 243 kbps Completed 19:04, 29 October 2023 1.0 s

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