English: Additional file 7: Video 5 Detection of intracellular Ca2+ signals with confocal microscopy - Control HepG2 cells. Nuclear and cytosolic Ca2+ were monitored in individual cells by using time-lapse confocal microscopy. HepG2 cells were cultured on glass coverslips in a density of 3×105 cells/well in 6 wells plates and kept in a Hepes-buffered solution during experiments. Cells were incubated with 4 μM cell permeant Fluo4-AM (fluo-4 acetoxymethyl ester; Molecular Probes) was added to the culture. ATP (10 μm) was used to trigger intracellular calcium signal.