English: Movie of the annotations from the NR database displayed by year (1993–2005). The movie tracks correctly annotated and misannotated sequences in the test set over the years 1993–2005. The similarity network is arranged by superfamily and colored as in figure 1 , i.e. all nodes of the same color were annotated to the same function. The network was generated from an all-by-all BLAST analysis of the test sequences with results that had BLAST E-value scores of 1×10−30 or lower retained. Nodes represent sequences deposited into the NR database during the years 1993–2005. Any two nodes are connected by an edge if at least one node found the other with a BLAST E-value less than or equal to 1×10−30. The network is visualized using Cytoscape v2.6.0-beta. The distance between any two connected nodes is roughly inversely proportional to the strength of the E-value between them (force-directed layout). The shapes of the nodes indicate annotation status: circles depict correctly annotated sequences and triangles depict incorrectly annotated sequences. Black arrows indicate examples in the haloacid dehalogenase family (HAD) and glyoxalase I family (VOC) that display potential evidence of error propagation. As these BLAST analyses were performed using a custom sequence database the resulting E-values are not necessarily comparable to the E-vaules determined by BLASTing against databases with large background models such as GenBank NR [60] .