English: "(A) Representative ABR waveforms from control (black), efavirenz-treated (red), and efavirenz-treated plus Phy (blue) mice recorded at 16 kHz tone bursts at increasing sound pressure levels (dB SPL). Thresholds are indicated by dashed traces and were determined by the presence of peak 1. Scale bar applies to the 3 recordings. ABR thresholds showed a slight increase in those mice treated with efavirenz that partially recovered when supplemented with Phy. Mean ABR thresholds (B) and ABR peak 1 (P1) amplitudes at 80 dB SPL (C) for control (n = 6), treated with efavirenz (n = 6), and treated with efavirenz together with Phy (n = 6) mice at different test frequencies. We included controls at 12 weeks; the age after the treatment (black; n = 6) and controls at 8 weeks, before starting the treatment (gray; n = 6). (D) DPOAEs thresholds for control (12 weeks; black; n = 6 and 8 weeks; gray; n = 6), treated with efavirenz (n = 6), and treated with efavirenz together with Phy (n = 6) mice at different test frequencies. DPOAEs thresholds showed a significant increase in those mice treated with efavirenz that partially recovered when supplemented with Phy. (E) Mean DPOAEs amplitudes versus level functions with f2 = 16 kHz in the 3 groups of mice. Group means ± SEM are shown (B-E). (F) Examples of fast Fourier transform traces showing the cubic DPOAE amplitude (2f1-f2), at f1 = 13.34 kHz; f2 = 16 kHz with f2 input level of 80 dB SPL. Scale bar of 50 dB SPL applies to the 3 recordings. Asterisks represent the statistical significance (Kruskal–Wallis test, followed by Dunn’s post-tests * = p < 0.05). The data underlying this figure can be found at https://osf.io/xpemk/. ABR, auditory brainstem response; Phy, phytosterols; SPL, sound pressure level."