File:Design, construction, and functional characterization of a tRNA neochromosome in yeast (graphical abstract).jpg
![File:Design, construction, and functional characterization of a tRNA neochromosome in yeast (graphical abstract).jpg](https://upload.wikimedia.org/wikipedia/commons/thumb/7/73/Design%2C_construction%2C_and_functional_characterization_of_a_tRNA_neochromosome_in_yeast_%28graphical_abstract%29.jpg/600px-Design%2C_construction%2C_and_functional_characterization_of_a_tRNA_neochromosome_in_yeast_%28graphical_abstract%29.jpg?20240304181910)
Size of this preview: 600 × 600 pixels. Other resolutions: 240 × 240 pixels | 480 × 480 pixels | 996 × 996 pixels.
Original file (996 × 996 pixels, file size: 106 KB, MIME type: image/jpeg)
File information
Structured data
Captions
Captions
"Designed, built, and characterized a neochromosome with 275 tRNA genes in yeast" "Functional analysis reveals insights into tRNA and chromosomal biology"
Summary
editDescriptionDesign, construction, and functional characterization of a tRNA neochromosome in yeast (graphical abstract).jpg |
English: "Here, we report the design, construction, and characterization of a tRNA neochromosome, a designer chromosome that functions as an additional, de novo counterpart to the native complement of Saccharomyces cerevisiae. Intending to address one of the central design principles of the Sc2.0 project, the ∼190-kb tRNA neochromosome houses all 275 relocated nuclear tRNA genes. To maximize stability, the design incorporates orthogonal genetic elements from non-S. cerevisiae yeast species. Furthermore, the presence of 283 rox recombination sites enables an orthogonal tRNA SCRaMbLE system. Following construction in yeast, we obtained evidence of a potent selective force, manifesting as a spontaneous doubling in cell ploidy. Furthermore, tRNA sequencing, transcriptomics, proteomics, nucleosome mapping, replication profiling, FISH, and Hi-C were undertaken to investigate questions of tRNA neochromosome behavior and function. Its construction demonstrates the remarkable tractability of the yeast model and opens up opportunities to directly test hypotheses surrounding these essential non-coding RNAs." |
Date | |
Source | https://www.cell.com/cell/fulltext/S0092-8674(23)01130-3 |
Author |
Authors of the study: Daniel Schindler Roy S.K. Walker Shuangying Jiang Aaron N. Brooks Yun Wang Carolin A. Müller Charlotte Cockram Yisha Luo Alicia García Daniel Schraivogel Julien Mozziconacci Noah Pena Mahdi Assari María del Carmen Sánchez Olmos Yu Zhao Alba Ballerini Benjamin A. Blount Jitong Cai Lois Ogunlana Wei Liu Katarina Jönsson Dariusz Abramczyk Eva Garcia-Ruiz Tomasz W. Turowski Reem Swidah Tom Ellis Tao Pan Francisco Antequera Yue Shen Conrad A. Nieduszynski Romain Koszul Junbiao Dai Lars M. Steinmetz Jef D. Boeke Yizhi Cai |
Licensing
edit![w:en:Creative Commons](https://upload.wikimedia.org/wikipedia/commons/thumb/7/79/CC_some_rights_reserved.svg/90px-CC_some_rights_reserved.svg.png)
![attribution](https://upload.wikimedia.org/wikipedia/commons/thumb/1/11/Cc-by_new_white.svg/24px-Cc-by_new_white.svg.png)
This file is licensed under the Creative Commons Attribution 4.0 International license.
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 18:19, 4 March 2024 | ![]() | 996 × 996 (106 KB) | Prototyperspective (talk | contribs) | Uploaded a work by Authors of the study: Daniel Schindler Roy S.K. Walker Shuangying Jiang Aaron N. Brooks Yun Wang Carolin A. Müller Charlotte Cockram Yisha Luo Alicia García Daniel Schraivogel Julien Mozziconacci Noah Pena Mahdi Assari María del Carmen Sánchez Olmos Yu Zhao Alba Ballerini Benjamin A. Blount Jitong Cai Lois Ogunlana Wei Liu Katarina Jönsson Dariusz Abramczyk Eva Garcia-Ruiz Tomasz W. Turowski Reem Swidah Tom Ellis Tao Pan Francisco Antequera Yue Shen Conrad A. Nieduszynski R... |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.