File:Differential-Effects-of-TNF-(TNFSF2)-and-IFN-γ-on-Intestinal-Epithelial-Cell-Morphogenesis-and-pone.0022967.s003.ogv
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editDescriptionDifferential-Effects-of-TNF-(TNFSF2)-and-IFN-γ-on-Intestinal-Epithelial-Cell-Morphogenesis-and-pone.0022967.s003.ogv |
English: Three-dimensional reconstruction of a T84 sphere. Cells were cultured for 7 days in matrigel to form hollow spheres. Cells were processed for immunolabeling with antibodies against the adherens and tight junction proteins β-catenin (in red) and ZO-1 (in green), respectively. Confocal stacks (over sampled) were generated, deconvoluted, 3D reconstructed as described in Materials and Methods. An animation of one 3D reconstructed luminal T84 sphere is shown. |
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Source | Movie S1 from Juuti-Uusitalo K, Klunder L, Sjollema K, Mackovicova K, Ohgaki R, Hoekstra D, Dekker J, van IJzendoorn S (2011). "Differential Effects of TNF (TNFSF2) and IFN-γ on Intestinal Epithelial Cell Morphogenesis and Barrier Function in Three-Dimensional Culture". PLOS ONE. DOI:10.1371/journal.pone.0022967. PMID 21853060. PMC: 3154921. | ||
Author | Juuti-Uusitalo K, Klunder L, Sjollema K, Mackovicova K, Ohgaki R, Hoekstra D, Dekker J, van IJzendoorn S | ||
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current | 18:23, 17 November 2012 | 6.4 s, 836 × 736 (1.25 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Juuti-Uusitalo K, Klunder L, Sjollema K, Mackovicova K, Ohgaki R, Hoekstra D, Dekker J, van IJzendoorn S |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Three-dimensional reconstruction of a T84 sphere. Cells were cultured for 7 days in matrigel to form hollow spheres. Cells were processed for immunolabeling with antibodies against the adherens and tight junction proteins ?-catenin (in red) and ZO-1 (in green), respectively. Confocal stacks (over sampled) were generated, deconvoluted, 3D reconstructed as described in Materials and Methods. An animation of one 3D reconstructed luminal T84 sphere is shown. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011 |