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File:Direct-Observation-of-Strand-Passage-by-DNA-Topoisomerase-and-Its-Limited-Processivity-pone.0034920.s005.ogv

Direct-Observation-of-Strand-Passage-by-DNA-Topoisomerase-and-Its-Limited-Processivity-pone.0034920.s005.ogv ‎(Ogg Theora video file, length 1 min 1 s, 220 × 156 pixels, 93 kbps, file size: 691 KB)

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English: Unbraiding of a 30(29.5)-turn DNA braid in two bursts. The movie consists of three portions (initial setup, first unbraiding burst, second burst resulting in complete unbraiding) as indicated by green horizontal bars in Figure 1C in the main text, with 1-s blackouts in between. The time displayed on the lower right matches that in Figure 1C. The white bar that appears at time 0 indicates 1.8 µm, the initial braid length. Note that the stage, and thus the DNA roots, were slightly moved to the right between ∼70 and ∼75 s to increase the tension (Figure 1D). The movie taken at 30 frames/s has been running-averaged for 30 frames, and the intensity adjusted to highlight DNA (bead images are saturated).
Date
Source Video S1 from Yogo K, Ogawa T, Hayashi M, Harada Y, Nishizaka T, Kinosita K (2012). "Direct Observation of Strand Passage by DNA-Topoisomerase and Its Limited Processivity". PLOS ONE. DOI:10.1371/journal.pone.0034920. PMID 22496876. PMC: 3322154.
Author Yogo K, Ogawa T, Hayashi M, Harada Y, Nishizaka T, Kinosita K
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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Date/TimeThumbnailDimensionsUserComment
current16:38, 19 November 20121 min 1 s, 220 × 156 (691 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 60 kbps Completed 22:36, 16 December 2023 7.0 s
WebM 360P 63 kbps Completed 04:52, 30 November 2023 3.0 s
Streaming 144p (MJPEG) 694 kbps Completed 09:47, 3 November 2023 2.0 s

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