File:Dorsal Root Galglia Neurons.tif
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English: These cosmic thingies are the spinal ganglia (bulb with ~ 10,000 neurons) with radially growing axons. They were extracted from the spinal cord of a newborn rat. They grew on a special flat scaffold (polycaprolactone), were fed for 7 days, and finally sacrificed in the name of science (by using formaldehyde solution). Eventually, they underwent immunostaining using three fluorophores (DAPI, S100, anti-beta3-tubulin), and final scanning with imaging via fluorescence Olympus microscope.
This work was performed at the Centre for Cell Engineering, University of Glasgow, Scotland, UK.
Українська: Ось ці космічні штуковини - спінальні нервові вузли, або ганглії (центральна бульба з ~ 10 000 нейронами), з радіально виходячими з них аксонами, экстраговані зі спинного мозку новонароджених щурів (2-3 дні). Були посаджені на спеціальну плоску підкладку (полікапролактон), годовані протягом 7 днів, після чого були вбиті розчином формальдегіду в ім'я науки. Пройшли чотирьохденну стадію імунофарбування з використанням трьох флуорофорів (DAPI, S100, anti-beta3-tubulin), потім були сфотографовані за допомогою методу флуоресцентної мікроскопії (Olympus).
Робота була виконана в Центрі Клітинної Iнженерії Університету Глазго (Шотландія, Велика Британія). English: DRG Neurons |
| Date | |
| Source | Own work |
| Author | Kseniia Bondarenko |
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This image was uploaded as part of Science Photo Competition 2016 |
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| current | 20:09, 28 October 2016 |  | 6,144 × 1,920 (8.55 MB) | Morne Arin (talk | contribs) | User created page with UploadWizard |
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