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File:Experimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s005.ogv

Experimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s005.ogv ‎(Ogg Theora video file, length 14 s, 694 × 520 pixels, 460 kbps, file size: 793 KB)

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English: Time-lapse imaging of IB formation and UPS dysfunction in U87MG cells transfected with HttQ103YFP-pIRES-mRFPu. Live cell imaging was initiated at 24 hours post-transfection and images acquired every 10 minutes. Cells were visualized using filters that detect YFP or RFP. Fluorescent images were merged to create a movie file. Images were acquired using a 10× objective for a total of 24 hours. At the beginning of the movie HttQ103 is expressed throughout the cell. At 36 hours (the half way point) many cells have formed an inclusion body and have notable accumulation of the red reporter protein (indicative of proteasome inhibition). Note that based on their morphology many cells with IB appear to be viable at the end of the movie.
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Source Video S2 from Tang M, Proctor C, Woulfe J, Gray D (2010). "Experimental and Computational Analysis of Polyglutamine-Mediated Cytotoxicity". PLOS Computational Biology. DOI:10.1371/journal.pcbi.1000944. PMID 20885783. PMC: 2944785.
Author Tang M, Proctor C, Woulfe J, Gray D
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Date/TimeThumbnailDimensionsUserComment
current21:55, 17 November 201214 s, 694 × 520 (793 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 152 kbps Completed 07:16, 26 August 2018 5.0 s
Streaming 480p (VP9) 152 kbps Completed 02:47, 7 February 2024 1.0 s
VP9 360P 89 kbps Completed 07:16, 26 August 2018 4.0 s
Streaming 360p (VP9) 89 kbps Completed 23:19, 13 March 2024 1.0 s
VP9 240P 57 kbps Completed 07:15, 26 August 2018 4.0 s
Streaming 240p (VP9) 57 kbps Completed 03:27, 21 December 2023 2.0 s
WebM 360P 473 kbps Completed 21:58, 17 November 2012 6.0 s
Streaming 144p (MJPEG) 392 kbps Completed 16:28, 10 November 2023 1.0 s

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