File:Experimental technique Dip-c.jpg
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Experimental chromatin conformation capture technique, Dip-c (Modified from Tan et al., 2021).
Summary edit
| Description |
Español: Primero, se extraen los núcleos celulares y se fijan. A continuación, se lleva a cabo una digestión enzimática de la cromatina usando enzimas de restricción y se ligan los productos próximos en el espacio empleando ligasas. Se amplifica el genoma completo (WGA) y se secuencia. Los resultados de la secuenciación permiten generar mapas de contactos cromosómicos y determinar la estructura 3D del genoma.
Tan, L. (2021). Determining the 3D genome structure of a single mammalian cell with dip-C. STAR Protocols, 2(3), 100622. doi:10.1016/j.xpro.2021.100622
English: First, cell nuclei are removed and fixed. Next, the chromatin is enzymatically digested using restriction enzymes and the proximate products are ligated in space using ligases. The whole genome is amplified (WGA) and sequenced. The sequencing results allow chromosome contact maps to be generated and the 3D structure of the genome to be determined.
Tan, L. (2021). Determining the 3D genome structure of a single mammalian cell with dip-C. STAR Protocols, 2(3), 100622. doi:10.1016/j.xpro.2021.100622
Deutsch: Zuerst werden Zellkerne entfernt und fixiert. Als nächstes wird das Chromatin enzymatisch unter Verwendung von Restriktionsenzymen verdaut und die proximaten Produkte werden im Raum unter Verwendung von Ligasen gebunden. Das gesamte Genom wird amplifiziert (WGA) und sequenziert. Die Sequenzierungsergebnisse ermöglichen die Generierung von Chromosomenkontaktkarten und die Bestimmung der 3D-Struktur des Genoms.
Tan, L. (2021). Determining the 3D genome structure of a single mammalian cell with dip-C. STAR Protocols, 2(3), 100622. doi:10.1016/j.xpro.2021.100622 |
| Date | |
| Source | Own work |
| Author | Andralgo |
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