File:FLIM-mKO-mCherry.png
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English: Example for Fluorescence lifetime imaging microscopy (FLIM). FLIM images of cells expressing mKO-p65 in absence (a–c) or presence (d–e) of mCherry-p65. The panels show the fluorescence intensity of two merged representative nuclei (a, d), the modulation lifetime map (b, e) and the histogram of the modulation lifetime distribution (e, f). The reduced lifetime observed for cells expressing both mKO-p65 and p65-mCherry is due to FRET, indicating homodimerization of p65. The width of the images corresponds to 28 µm. Figure derived from Goedhart J, Vermeer JE, Adjobo-Hermans MJ, van Weeren L, Gadella TW (2007). "Sensitive detection of p65 homodimers using red-shifted and fluorescent protein-based FRET couples". PLoS ONE 2 (10): e1011. DOI:10.1371/journal.pone.0001011. PMID 17925859. PMC: 1995760. |
| Date | |
| Source | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0001011 |
| Author | Joachim Goedhart, Joop E. M. Vermeer, Merel J. W. Adjobo-Hermans, Laura van Weeren, Theodorus W. J. Gadella Jr., modified by S. Jähnichen (talk) |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 20:52, 3 April 2010 | | 996 × 1,107 (682 KB) | S. Jähnichen (talk | contribs) | {{Information |Description={{en|1=Example for Fluorescence lifetime imaging microscopy (FLIM). FLIM images of cells expressing mKO-p65 in absence (a–c) or presence (d–e) of mCherry-p65. The panels show the fluorescence intensity of two merged represen |
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