English: Expression levels of genes flanking the T-DNA/vector insert. (A) Relative expression levels of At1g13020 (dark gray bars) and At1g21550 (light gray bars) in wild-type plants (WT) and in sns-D mutants with weak and strong phenotypes as determined via Q-RT-PCR. The expression level in the wild-type was set on 1 and the SD in the mutants was indicated by error bars. (B) Northern blot analysis of At1g13020 in the wild-type (W) and the sns-D mutant (S) at different growth stages. G, just germinated; 1, 1 week after germination; 2, 2 weeks after germination; 3, 3 weeks after germination; 4, 4 weeks after germination; 5, 5 weeks after germination; Sw, sns-D plants with weak phenotype; Ss, sns-D plants with strong phenotype. Loading of RNA was determined by quantification of the bands after hybridization with At4g38740 (cyclophilin). Relative expression of At1g13020 in the mutant plants was shown for each developmental stage. The expression in the wild-type was set on 1.