English: Vicia faba L., minor cv. Scirocco (Saaten-Union GmbH, Isernhagen, Germany). Excessive water exits the apoplast through the stomata. Bright-field images of the adaxial leaf face (gray-scaled; A-i, B-i, and C-i) and corresponding pseudo-green fluorescence images at ex 495 nm (A-ii, B-ii, and C-ii). Images were collected (A) immediately after dye loading and at (B) 2 min and (C) 10 min after loading. (A-i, -ii) Immediately after loading, no water droplets are found to be located onto the stomata. (B-i) Water droplets that had left the apoplast through the stomata 2 min after dye insertion are indicated by #. (B-ii) The corresponding fluorescence image at ex 495 nm reveals that these droplets did not contain any dye, as otherwise fluorescence signals would be emitted at ex 495 nm and appear in pseudo-green. (C-i and C-ii) After 10 min, more water had left the apoplastic space as indicated by #. a, stomatal cavity; b, palisade cell.