English: Microarray design and transcriptome size in UV-B-irradiated and shielded leaves and immature ears after different UV-B irradiation times. (A) Microarray hybridization design with direct comparisons to measure UV-B effects on signaling initiation from irradiated (irradiated leaves, IL) to shielded tissues (shielded leaves, SL; and immature ears, IE). All time course treatments had two leaves exposed above the PE. Each arrow represents a pair of samples hybridized on one array; in every case there is one cy3-labeled sample compared to one cy5-labeled sample. For each sample type there were four biological replicates, generating at least four hybridization comparisons, except where noted. No UV-B irradiation or control (0 min or C) plants did not receive any UV-B, see Materials and Methods for details of the tissue sampling protocol. (B) Transcriptome size in IL and SL, and IE from plants that were irradiated with UV-B in the two upper leaves after 10, 30, and 60 min of exposure, and control plants that did not receive any UV-B (0 min, C).