File:GFP Superresolution Christoph Cremer.JPG
GFP_Superresolution_Christoph_Cremer.JPG (538 × 389 pixels, file size: 156 KB, MIME type: image/jpeg)
Captions
Contents
Summary
editDescriptionGFP Superresolution Christoph Cremer.JPG |
GFP superresolution, optical nanoscopy ( Christoph Cremer, emeritus at Heidelberg university [1]) View of a nucleus of a bone cancer cell: using normal high resolution fluorescence microscopy, it is not possible to distinguish details of its structure (image on the left). Using the two Color Localization Microscopy 2CLM (image on the right) it is possible to localize 70,000 histone molecules (red: RFP-H2A) and 50,000 chromatin remodeling proteins (green: GPF-Snf2H) in a field of view of 470 µm2 with an optical depth of 600 nm. Common fluorescence markers were used. 2CLM is the only optical nanoscopy method that allows position based co-localization of single molecules at high density in a wide field of view using conventional fluorescent proteins such as GFP, YFP, RFP, or other conventional fluorochromes. Due to its high optical single molecule resolution, 2CLM allows significantly more precise analyses of potential protein interactions than FRET-(Fluorescence Resonance Energy Transfer) technology, which is at present the preferred method for such investigations. This is of particular significance in studies of biomolecular machines (BMMs) within cells: Single BMMS can be analysed, including the number of molecules of a given type; distances between proteins in these BMMs often are substantially greater than those that can be analyzed by FRET (restricted to a maximum distance of only a few nm). Possible to use conventional, well established and inexpensive fluorescent dyes, from the GFP group, and its dye variants, to the well-known Alexa and fluorescein dyes. Fundamental to SPDMphymod are blinking phenomena (flashes of fluorescence), induced by reversible bleaches (metastable dark states). Individual molecules of the same spectral emission color can be detected. Publikation: Manuel Gunkel, Fabian Erdel, Karsten Rippe, Paul Lemmer, Rainer Kaufmann, Christoph Hörmann, Roman Amberger and Christoph Cremer: Dual color localization microscopy of cellular nanostructures. In: Biotechnology Journal, 2009, 4, 927-938. ISSN 1860-6768 |
||
Date | 073009 | ||
Source | Own work | ||
Author | Andy Nestl | ||
Permission (Reusing this file) |
|
Gallery
edit-
Breast Cancer Cells: 3D Dual Color Super Resolution Microscopy of Her2 and Her3 & cluster calculations
-
Single YFP molecule detection in a human cancer cell. Typical distance measurements 15 nm
-
Co- localisation microscopy with GFP and RFP fusion proteins (nucleus of a bone cancer cell) 120.000 localized molecules in a widefield area(470 µm2)
-
Label-free Localisation Microscopy SPDM - Super Resolution Microscopy reveals prior undetebable intracellular structures
-
Investigation of human eye tissue, affected by macular degeneration AMD
-
Virus Super Resolution Microscopy SPDM Cremer/Wege labs
Licensing
edit- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- share alike – If you remix, transform, or build upon the material, you must distribute your contributions under the same or compatible license as the original.
Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled GNU Free Documentation License.http://www.gnu.org/copyleft/fdl.htmlGFDLGNU Free Documentation Licensetruetrue |
Summary
edit- ↑ https://www.physik.uni-heidelberg.de/personen/lsf.php?details=1537 |titel=Fakultät für Physik und Astronomie |abruf=2020-10-01
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 12:14, 30 July 2009 | 538 × 389 (156 KB) | Andy Nestl (talk | contribs) | {{Information |Description=GFP superresolution, optical nanoscopy (Christoph Cremer) |Source=Own work by uploader |Date=073009 |Author=Andy Nestl |Permission=given by Christoph Cremer, University of Heidelberg |other_versions= }} |
You cannot overwrite this file.
File usage on Commons
The following 6 pages use this file:
- File:3D Dual Color Super Resolution Microscopy Cremer 2010.png
- File:GFP Superresolution Christoph Cremer.JPG
- File:Label-free Localisation Microscopy SPDM - Super Resolution Microscopy Christoph Cremer.jpg
- File:Opthalmology AMD Super Resolution Cremer.png
- File:Single YFP molecule superresolution microscopy.png
- File:TMV virus super resolution microscopy Christoph Cremer Christina Wege.jpg
File usage on other wikis
The following other wikis use this file:
- Usage on ar.wikipedia.org
- Usage on be.wikipedia.org
- Usage on bn.wikipedia.org
- Usage on ca.wikipedia.org
- Usage on cs.wikipedia.org
- Usage on de.wikipedia.org
- Usage on en.wikipedia.org
- Usage on en.wikibooks.org
- Usage on eo.wikipedia.org
- Usage on fa.wikipedia.org
- Usage on fr.wikipedia.org
- Usage on gl.wikipedia.org
- Usage on he.wikipedia.org
- Usage on it.wikipedia.org
- Usage on mai.wikipedia.org
- Usage on ne.wikipedia.org
- Usage on nl.wikipedia.org
- Usage on pl.wikipedia.org
- Usage on pt.wikipedia.org
- Usage on sv.wikipedia.org
- Usage on ta.wikipedia.org
- Usage on vi.wikipedia.org
- Usage on zh.wikipedia.org
Metadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
_error | 0 |
---|