File:Mapping-the-dynamics-and-nanoscale-organization-of-synaptic-adhesion-proteins-using-monomeric-ncomms10773-s7.ogv

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English: Supplementary Movie 6 Dual-color imaging of neurexin-1β and neuroligin-1 at synapses. Neurons electroporated with either Homer1c-GFP, AP-Nlg1, and BirAER, or BFP-Nrx1β, were plated together and cultured until DIV15. AP-Nlg1 was labeled with mSA-Atto 594, and BFP-Nrx1β with Atto-647N-nanobody, simultaneously on the imaging setup. Sequential acquisitions of mSA-Atto 594 and Atto 647N-nanobody were performed by uPAINT, and superimposed here for a time period of 20 s (400 planes of 50 ms each). The movie represents the overlay of mSA-Atto594 (red), Atto 647N-nanobody (green), and Homer1c-GFP (white). The dotted line shows the contour of a dendrite expressing Homer1c-GFP, AP-Nlg1, and BirAER.
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Source Video file from Chamma I, Letellier M, Butler C, Tessier B, Lim K, Gauthereau I, Choquet D, Sibarita J, Park S, Sainlos M, Thoumine O (2016). "Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin". Nature Communications. DOI:10.1038/ncomms10773. PMID 26979420. PMC: 4799371.
Author Chamma I, Letellier M, Butler C, Tessier B, Lim K, Gauthereau I, Choquet D, Sibarita J, Park S, Sainlos M, Thoumine O
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Date/TimeThumbnailDimensionsUserComment
current17:12, 28 October 201620 s, 1,153 × 220 (2.27 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 480P 279 kbps Completed 19:33, 2 September 2018 12 s
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VP9 360P 176 kbps Completed 19:33, 2 September 2018 7.0 s
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VP9 240P 113 kbps Completed 19:33, 2 September 2018 8.0 s
Streaming 240p (VP9) 113 kbps Completed 07:43, 2 February 2024 1.0 s
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Streaming 144p (MJPEG) 845 kbps Completed 21:12, 10 November 2023 2.0 s

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