File:Mapping-the-dynamics-and-nanoscale-organization-of-synaptic-adhesion-proteins-using-monomeric-ncomms10773-s7.ogv
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DescriptionMapping-the-dynamics-and-nanoscale-organization-of-synaptic-adhesion-proteins-using-monomeric-ncomms10773-s7.ogv |
English: Supplementary Movie 6 Dual-color imaging of neurexin-1β and neuroligin-1 at synapses. Neurons electroporated with either Homer1c-GFP, AP-Nlg1, and BirAER, or BFP-Nrx1β, were plated together and cultured until DIV15. AP-Nlg1 was labeled with mSA-Atto 594, and BFP-Nrx1β with Atto-647N-nanobody, simultaneously on the imaging setup. Sequential acquisitions of mSA-Atto 594 and Atto 647N-nanobody were performed by uPAINT, and superimposed here for a time period of 20 s (400 planes of 50 ms each). The movie represents the overlay of mSA-Atto594 (red), Atto 647N-nanobody (green), and Homer1c-GFP (white). The dotted line shows the contour of a dendrite expressing Homer1c-GFP, AP-Nlg1, and BirAER. |
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Source | Video file from Chamma I, Letellier M, Butler C, Tessier B, Lim K, Gauthereau I, Choquet D, Sibarita J, Park S, Sainlos M, Thoumine O (2016). "Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin". Nature Communications. DOI:10.1038/ncomms10773. PMID 26979420. PMC: 4799371. | ||
Author | Chamma I, Letellier M, Butler C, Tessier B, Lim K, Gauthereau I, Choquet D, Sibarita J, Park S, Sainlos M, Thoumine O | ||
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Short title | Supplementary Movie 6 |
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Author | Chamma I, Letellier M, Butler C, Tessier B, Lim K, Gauthereau I, Choquet D, Sibarita J, Park S, Sainlos M, Thoumine O |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Dual-color imaging of neurexin-1β and neuroligin-1 at synapses. Neurons electroporated with either Homer1c-GFP, AP-Nlg1, and BirAER, or BFP-Nrx1β, were plated together and cultured until DIV15. AP-Nlg1 was labeled with mSA-Atto 594, and BFP-Nrx1β with Atto-647N-nanobody, simultaneously on the imaging setup. Sequential acquisitions of mSA-Atto 594 and Atto 647N-nanobody were performed by uPAINT, and superimposed here for a time period of 20 s (400 planes of 50 ms each). The movie represents the overlay of mSA-Atto594 (red), Atto 647N-nanobody (green), and Homer1c-GFP (white). The dotted line shows the contour of a dendrite expressing Homer1c-GFP, AP-Nlg1, and BirAER. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-03-16 |