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File:Mitochondrial-and-Plasma-Membrane-Pools-of-Stomatin-Like-Protein-2-Coalesce-at-the-Immunological-pone.0037144.s001.ogv

Mitochondrial-and-Plasma-Membrane-Pools-of-Stomatin-Like-Protein-2-Coalesce-at-the-Immunological-pone.0037144.s001.ogv ‎(Ogg Theora video file, length 12 s, 520 × 512 pixels, 1.25 Mbps, file size: 1.78 MB)

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English: Localization of SLP-2 during IS formation. Antigen presenting cells (APCs) were incubated overnight with SEE (1 µg/ml), washed, resuspended in medium, and incubated on polylysine-coated glass bottom microwell dishes for 10 minutes to allow cells to adhere. SLP-2-gfp-expressing Jurkat T cells were added to the APCs preincubated with SEE on the polylysine-coated microwell dishes. Once T:APC doublet was identified, images were acquired every 30 seconds for 60 minutes. The majority of SLP-2-gfp localized proximal to the synapse and distributed evenly in early stages, and as the IS matures SLP-2-gfp moved to periphery of the synapse.
Date
Source Video S1 from Christie D, Kirchhof M, Vardhana S, Dustin M, Madrenas J (2012). "Mitochondrial and Plasma Membrane Pools of Stomatin-Like Protein 2 Coalesce at the Immunological Synapse during T Cell Activation". PLOS ONE. DOI:10.1371/journal.pone.0037144. PMID 22623988. PMC: 3356372.
Author Christie D, Kirchhof M, Vardhana S, Dustin M, Madrenas J
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Date/TimeThumbnailDimensionsUserComment
current21:20, 30 October 201212 s, 520 × 512 (1.78 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 469 kbps Completed 14:10, 3 September 2018 8.0 s
Streaming 480p (VP9) Not ready Unknown status
VP9 360P 295 kbps Completed 14:10, 3 September 2018 7.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 180 kbps Completed 14:10, 3 September 2018 5.0 s
Streaming 240p (VP9) 180 kbps Completed 05:33, 3 February 2024 0.0 s
WebM 360P 512 kbps Completed 10:51, 20 November 2012 9.0 s
Streaming 144p (MJPEG) 464 kbps Completed 03:26, 13 November 2023 1.0 s

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