File:Mitochondrial-and-Plasma-Membrane-Pools-of-Stomatin-Like-Protein-2-Coalesce-at-the-Immunological-pone.0037144.s001.ogv
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Mitochondrial-and-Plasma-Membrane-Pools-of-Stomatin-Like-Protein-2-Coalesce-at-the-Immunological-pone.0037144.s001.ogv (Ogg Theora video file, length 12 s, 520 × 512 pixels, 1.25 Mbps, file size: 1.78 MB)
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DescriptionMitochondrial-and-Plasma-Membrane-Pools-of-Stomatin-Like-Protein-2-Coalesce-at-the-Immunological-pone.0037144.s001.ogv |
English: Localization of SLP-2 during IS formation. Antigen presenting cells (APCs) were incubated overnight with SEE (1 µg/ml), washed, resuspended in medium, and incubated on polylysine-coated glass bottom microwell dishes for 10 minutes to allow cells to adhere. SLP-2-gfp-expressing Jurkat T cells were added to the APCs preincubated with SEE on the polylysine-coated microwell dishes. Once T:APC doublet was identified, images were acquired every 30 seconds for 60 minutes. The majority of SLP-2-gfp localized proximal to the synapse and distributed evenly in early stages, and as the IS matures SLP-2-gfp moved to periphery of the synapse. |
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Source | Video S1 from Christie D, Kirchhof M, Vardhana S, Dustin M, Madrenas J (2012). "Mitochondrial and Plasma Membrane Pools of Stomatin-Like Protein 2 Coalesce at the Immunological Synapse during T Cell Activation". PLOS ONE. DOI:10.1371/journal.pone.0037144. PMID 22623988. PMC: 3356372. | ||
Author | Christie D, Kirchhof M, Vardhana S, Dustin M, Madrenas J | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:20, 30 October 2012 | 12 s, 520 × 512 (1.78 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Christie D, Kirchhof M, Vardhana S, Dustin M, Madrenas J |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |