Wikimedia Commons

File:Monitoring-the-Cytoskeletal-EGF-Response-in-Live-Gastric-Carcinoma-Cells-pone.0045280.s005.ogv

Original file ‎(Ogg Theora video file, length 17 s, 752 × 976 pixels, 10.53 Mbps, file size: 21.29 MB)

Captions

Captions

Add a one-line explanation of what this file represents

Summary edit

Description
English: Triple labeling of MKN1 cells reveals sequential reorganization of cytoskeletal filaments in response to EGF. MKN1 were transfected with expression constructs for mRFP-actin, EB3-CFP and HK18-YFP. The composite Video displays images that were recorded every 38 s starting 10 min after addition of 30 ng/ml EGF. It depicts the individual fluorescence patterns of EB3-CFP (EB3), mRFP-actin (ACTB) and HK18-YFP (KRT18) first in inverse presentation separately (cell contours demarcated in orange), then as a merged overlays of EB3-CFP (false green color) and mRFP-actin (red), and finally as merged overlays of EB3-CFP (false green color), mRFP-actin (red) and HK18-YFP (false blue color) as indicated. Note that EGF-induced cell extensions are positive for actin and that dynamic microtubules enter these regions which contain very little keratin. Bar, 10 µm.
Date
Source Video S5 from Felkl M, Tomas K, Smid M, Mattes J, Windoffer R, Leube R (2012). "Monitoring the Cytoskeletal EGF Response in Live Gastric Carcinoma Cells". PLOS ONE. DOI:10.1371/journal.pone.0045280. PMID 23028903. PMC: 3459943.
Author Felkl M, Tomas K, Smid M, Mattes J, Windoffer R, Leube R
Permission
(Reusing this file)
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 2.5 Generic license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
PLOS
PLOS
This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
Provenance
InfoField
This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
WikiProject Open Access
WikiProject Open Access

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current16:39, 30 October 201217 s, 752 × 976 (21.29 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

You cannot overwrite this file.

There are no pages that use this file.

Transcode status

Update transcode status
Format Bitrate Download Status Encode time
VP9 720P 1.7 Mbps Completed 17:48, 3 September 2018 34 s
Streaming 720p (VP9) Not ready Unknown status
VP9 480P 852 kbps Completed 17:48, 3 September 2018 30 s
Streaming 480p (VP9) Not ready Unknown status
VP9 360P 428 kbps Completed 17:48, 3 September 2018 22 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 208 kbps Completed 17:48, 3 September 2018 18 s
Streaming 240p (VP9) 209 kbps Completed 08:07, 3 February 2024 1.0 s
WebM 360P 235 kbps Completed 08:13, 10 November 2012 17 s
Streaming 144p (MJPEG) 1.23 Mbps Completed 13:03, 13 November 2023 2.0 s

Metadata

This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.

Retrieved from "https://commons.wikimedia.org/w/index.php?title=File:Monitoring-the-Cytoskeletal-EGF-Response-in-Live-Gastric-Carcinoma-Cells-pone.0045280.s005.ogv&oldid=809461859"