File:Morphological character and molecular marker expression of adherent spheroid cells of HCT-116 and HT29.png
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Figure 1. Morphological character and molecular marker expression of adherent/ spheroid cells of HCT-116 and HT29. Both HCT116 and HT29 can form large round, unattached floating colonsphere of 50–100 mm when cultured in SFM. FCM analysis showed that CD133 expression rise from #1.2% up to 60%–80% when cultured in SFM. And strongly expression of Lgr5 was detected by immunofluorescence staining. But as the marker of differentiated epithelial cell, CK20 expression presented adverse change. doi:10.1371/journal.pone.0073341.g00 |
| Date | |
| Source | https://www.researchgate.net/figure/EMT-phenotype-detection-of-spheroid-adherent-cells-Representative-photomicrographs_fig8_256613796 https://www.researchgate.net/publication/256613796_Epithelial-Mesenchymal_Transition_Associates_with_Maintenance_of_Stemness_in_Spheroid-Derived_Stem-Like_Colon_Cancer_Cells Epithelial-Mesenchymal Transition Associates with Maintenance of Stemness in Spheroid-Derived Stem-Like Colon Cancer Cells. PLoS ONE 8(9): e73341. doi:10.1371/journal.pone.007334 |
| Author | Han X-Y, Wei B, Fang J-F, Zhang S, Zhang F-C, et al. |
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ß 2013 Han et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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| current | 10:05, 20 May 2024 | | 1,571 × 1,437 (1.14 MB) | Rasbak (talk | contribs) | {{Information |description=Figure 1. Morphological character and molecular marker expression of adherent/ spheroid cells of HCT-116 and HT29. Both HCT116 and HT29 can form large round, unattached floating colonsphere of 50–100 mm when cultured in SFM. FCM analysis showed that CD133 expression rise from #1.2% up to 60%–80% when cultured in SFM. And strongly expression of Lgr5 was detected by immunofluorescence staining. But as the marker of differentiated epithelial cell, CK20 expression prese... |
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