File:PGCs and their surrounding cells in the germinal crescent co-express transcripts for fibronectin and integrin beta 1. Quail.jpg
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| Description | Figure 9. PGCs and their surrounding cells in the germinal crescent co-express transcripts for fibronectin and integrin beta 1. mRNA for fibronectin, DAZL and integrin beta1 receptor subunit are localized to PGCs and their surrounding cells in both the extra-embryonic germinal crescent and in the embryo proper. Whole-mount multiplex hybridization chain reaction (HCR) for Fibronectin1 (FN1, red), Integrin beta1 subunit (ITGB1, blue), and DAZL (green) mRNA transcripts in HH4 quail embryos. All images are single optical sections (3.2 μm each) from 20x confocal Z stacks with 2x zoom. (A,B) GC = germinal crescent. (C,D) EMB = Embryo. Mesoderm/hypoblast in the mid-lateral region of the embryo containing PGCs that have not yet reached the germinal crescent. (B) Bounding box from A at higher magnification. (D) Bounding box from (C) at higher magnification. 20 μm scale bar in (B) also applies to (D). (E) AF = Auto-fluorescence control and (F) NSA = Non-specific amplification controls using the indicated laser lines. Control imaging conditions were identical to those used in (A–D). Twenty five micrometer scale bar in (E) also applies to (A,C,F). |
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| Source | https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2019.00035/full Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration. Front. Cell Dev. Biol. 7:35. doi: 10.3389/fcell.2019.00035 |
| Author | Huss DJ, Saias S, Hamamah S, Singh JM, Wang J, Dave M, Kim J, Eberwine J and Lansford R |
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© 2019 Huss, Saias, Hamamah, Singh, Wang, Dave, Kim, Eberwine and Lansford. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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| current | 10:18, 11 July 2024 | | 957 × 720 (680 KB) | Rasbak (talk | contribs) | {{Information |description=Figure 9. PGCs and their surrounding cells in the germinal crescent co-express transcripts for fibronectin and integrin beta 1. mRNA for fibronectin, DAZL and integrin beta1 receptor subunit are localized to PGCs and their surrounding cells in both the extra-embryonic germinal crescent and in the embryo proper. Whole-mount multiplex hybridization chain reaction (HCR) for Fibronectin1 (FN1, red), Integrin beta1 subunit (ITGB1, blue), and DAZL (green) mRNA transcripts... |
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