File:Presumptive primitive streak area of ROCK-inhibited embryos reveals abnormality of orientation of cell divisions as well as of actin distribution.jpg

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Development 142: doi:10.1242/dev.111583: Supplementary Material DEV111583 Supplementary Material Legends of Supplementary Figures Fig. S1. Presumptive primitive streak area of ROCK-inhibited embryos reveals abnormality of orientation of cell divisions as well as of actin distribution. (A) Number of cell divisions used for the orientation of cell divisions counted in three control (n=112) and three embryos treated with Y-27632 (n=58) in the time period of 100 min. (B,C) Orientation of metaphase plates in relation to the AP axis (0°-±180°) Development 142: doi:10.1242/dev.111583: Supplementary Material taken from the PGE area of control embryos (blue) and embryos treated with Y-27632 (red). Radial graduation shows the number of cells that fall within a specific angular region and orbital graduation shows the groups of angles (0°-±180°). (D,E) Dorsal views of control (D) and treated (E) embryo with high magnifications (D1-E3) of phalloidin-TRITC and DAPI staining in anterior (D1, E1) and posterior (D2,3, E2,3) regions (boxed in D and E). Clump-like actin distribution (foci) is found near the nucleus of epiblast cells in the presumptive primitive streak area of embryos treated

with Y-27632, only. Scale bars: D, E, 250 μm; D1-D3, E1-E3 10 μm
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Source https://journals.biologists.com/dev/article/142/1/92/46970/Rho-kinase-activity-controls-directional-cell Rho kinase activity controls directional cell movements during primitive streak formation in the rabbit embryo. Development. 2015 Jan 1;142(1):92-8. doi: 10.1242/dev.111583. PMID: 25516971; PMCID: PMC4299133.
Author Stankova V, Tsikolia N, Viebahn C.
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© 2015. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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current00:38, 29 April 2024Thumbnail for version as of 00:38, 29 April 20241,277 × 1,154 (921 KB)Rasbak (talk | contribs)== {{int:filedesc}} == {{Information |description=Development 142: doi:10.1242/dev.111583: Supplementary Material DEV111583 Supplementary Material Legends of Supplementary Figures Fig. S1. Presumptive primitive streak area of ROCK-inhibited embryos reveals abnormality of orientation of cell divisions as well as of actin distribution. (A) Number of cell divisions used for the orientation of cell divisions counted in three control (n=112) and three embryos treated with Y-27632 (n=58) in the tim...

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