English: Light sheet fluorescence microscopy (LSFM) splits fluorescence excitation and detection into two separate light paths, with the axis of illumination perpendicular to the detection axis. That means you illuminate a single thin section of the sample at one time, generating an inherent optical section by exciting only fluorescence from the in-focus plane. No pinhole or image processing is required. Light from the in-focus plane is collected on the pixels of a camera, rather than pixel by pixel as, for example, in confocal or other laser scanning microscopes. Parallelization of the image collection on a camera-based detector lets you collect images faster and with less excitation light than you would with many other microscope techniques. www.zeiss.com/lightsheet