File:Proposedmechanismforbglii.gif

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English: BglII catalyses phosphodiester bond cleavage at the DNA backbone through a phosphoryl transfer to water. Studies on the mechanism of restriction enzymes have revealed several general features that seem to be true in almost all cases, although the actual mechanism for each enzyme is most likely some variation of this general mechanism. This mechanism requires a base to generate the hydroxide ion from water, which will act as the nucleophile and attack the phosphorus in the phosphodiester bond. Also required is a Lewis acid to stabilize the extra negative charge of the pentacoordinated transition state phosphorus as well as a general acid or metal ion that stabilizes the leaving group (3’-O-).
Date 12/10/08
Source Own work
Author Gwilliams10

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current16:05, 17 December 2008Thumbnail for version as of 16:05, 17 December 2008909 × 334 (15 KB)Gwilliams10 (talk | contribs)
16:02, 17 December 2008Thumbnail for version as of 16:02, 17 December 2008470 × 175 (6 KB)Gwilliams10 (talk | contribs)
23:10, 11 December 2008Thumbnail for version as of 23:10, 11 December 20085,908 × 2,349 (150 KB)Gwilliams10 (talk | contribs)
23:08, 11 December 2008Thumbnail for version as of 23:08, 11 December 20085,908 × 2,349 (150 KB)Gwilliams10 (talk | contribs)
02:04, 11 December 2008Thumbnail for version as of 02:04, 11 December 2008696 × 290 (10 KB)Gwilliams10 (talk | contribs)
01:57, 11 December 2008Thumbnail for version as of 01:57, 11 December 2008750 × 317 (15 KB)Gwilliams10 (talk | contribs)
01:50, 11 December 2008Thumbnail for version as of 01:50, 11 December 2008538 × 228 (7 KB)Gwilliams10 (talk | contribs)
01:48, 11 December 2008Thumbnail for version as of 01:48, 11 December 2008700 × 307 (13 KB)Gwilliams10 (talk | contribs)
01:42, 11 December 2008Thumbnail for version as of 01:42, 11 December 2008466 × 205 (6 KB)Gwilliams10 (talk | contribs){{Information |Description= |Source= |Date= |Author= |Permission= |other_versions= }}
01:39, 11 December 2008Thumbnail for version as of 01:39, 11 December 2008467 × 472 (6 KB)Gwilliams10 (talk | contribs){{Information |Description={{en|1=Mechanism of BglII Restriction Endonuclease}} |Source=Own work by uploader |Author=Gwilliams10 |Date=12/10/08 |Permission= |other_versions= }} <!--{{ImageUpload|full}}-->

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