File:Replication fork restarts by homologous recombination following replication stress.png
Size of this preview: 600 × 600 pixels. Other resolutions: 240 × 240 pixels | 480 × 480 pixels | 768 × 768 pixels | 1,024 × 1,024 pixels | 1,700 × 1,700 pixels.
Original file (1,700 × 1,700 pixels, file size: 473 KB, MIME type: image/png)
File information
Structured data
Captions
Summary
editDescriptionReplication fork restarts by homologous recombination following replication stress.png |
English: (A) Model of repair of blocking lesions. (A.1) DNA adducts obstruct DNA synthesis by replicative DNA polymerases. Fork progression on a damaged template might involve a repriming event downstream of the damage, which leaves a ssDNA gap behind the moving fork. Rad51 then nucleates on the ssDNA gaps and promotes the recombination with the sister chromatid to seal the gap. Other mechanisms might be involved in the bypass of DNA lesions such as translesion synthesis (TLS). (A.2) Model of fork regression at a stalled fork: A slowing down of fork velocity or fork arrest leads to a transient uncoupling of the helicase and polymerases, thus exposing ssDNA at the stalled fork. The fork reversion forms a “chicken foot” structure (i.e., the fork and the nascent strand, which is complementary, being annealed together to form a four-way junction). Cleavage of this structure might involve MUS81 and leads to single-ended DSB formation. (B) Model of broken-fork repair. A replication fork can be converted into single-ended DSBs following the passage of the fork through a nick or following cleavage by an endonuclease. The single-ended break is then resected and Rad51 nucleates on the exposed ssDNA and promotes recombination with the sister chromatid. The 3' end of the invading strand primes DNA synthesis, and the replisome has been proposed to be rebuilt from the extended D-loop structure. (C) Model of fork restarts at a collapsed fork. Fork collapse might arise from a stalled fork where the replisome fails to be maintained in a functional state or when the replisome encounters physical obstacles such as tightly DNA bound proteins or RNA/DNA hybrids. Resection of nascent strands might help the fork to regress (i.e., the fork moving backward without the annealing of nascent strands) and thus allow the 3' end of the nascent strand to be extruded. Rad51 nucleates on the exposed ssDNA and promotes recombination with the parental DNA duplex. The replisome could again be rebuilt from the extended D-loop.[1] |
Date | |
Source | https://doi.org/10.3390/genes6020267 |
Author | Camille Gelot, Indiana Magdalou, and Bernard S. Lopez |
Licensing
editThis file is licensed under the Creative Commons Attribution 4.0 International license.
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 23:05, 24 May 2020 | 1,700 × 1,700 (473 KB) | Rob Hurt (talk | contribs) | Uploaded a work by Camille Gelot, Indiana Magdalou, and Bernard S. Lopez from https://doi.org/10.3390/genes6020267 with UploadWizard |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
File usage on other wikis
The following other wikis use this file:
- Usage on bs.wikipedia.org
- Usage on en.wikipedia.org
Metadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Image width | 1,700 px |
---|---|
Image height | 1,700 px |
Color space | sRGB |
Width | 1,700 px |
Height | 1,700 px |
File change date and time | 08:54, 24 May 2020 |
Date metadata was last modified | 08:54, 24 May 2020 |
Horizontal resolution | 157.48 dpi |
Vertical resolution | 157.48 dpi |