English: "a, Schematic diagram of FMT. Faecal samples from human donors after an 8-week RS or CS intervention (n = 4 per intervention) were transplanted to antibiotic (Abx)-treated C57BL/6 mice. The mice were fed an irradiated western diet (WD) for 2 weeks before and during the 14 d of colonization (n = 16 per group). b,c, Body weight and fat mass changes after FMT. d, Representative photographs of visceral fat in mice colonized for 14 d with microbiota from RS or CS donors. e, Adipose depot mass including inguinal subcutaneous fat (iSAT), eWAT, pWAT and mWAT in mice colonized for 14 d with microbiota. f,g, Representative images and quantification data of haematoxylin and eosin-stained sections of iSAT, eWAT and mWAT in the two groups of mice colonized with CS (top) or RS microbiota (bottom). Scale bar, 100 µm. Data were reproduced in three independent experiments. Data are presented as mean ± s.e.m. Significance was determined by paired two-tailed Student’s t-test (c) and unpaired two-tailed Student’s t-test (b,e,g) (normally distributed) or nonparametric two-sided Wilcoxon rank-sum test (non-normally distributed). *P = 0.02 and 0.01 (g), **P = 0.002 (b), ***P < 0.001 (b,c,e). Source data"