File:Slowing-of-axonal-regeneration-is-correlated-with-increased-axonal-viscosity-during-aging-1471-2202-11-140-S1.ogv
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Slowing-of-axonal-regeneration-is-correlated-with-increased-axonal-viscosity-during-aging-1471-2202-11-140-S1.ogv (Ogg Theora video file, length 4.0 s, 342 × 365 pixels, 825 kbps, file size: 403 KB)
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DescriptionSlowing-of-axonal-regeneration-is-correlated-with-increased-axonal-viscosity-during-aging-1471-2202-11-140-S1.ogv |
English: Movement of the axonal framework occurs during normal axonal elongation of neonatal sensory neurons. This movie demonstrates that mitochondria docked to the axonal framework advance forward during normal axonal elongation. Sensory neurons from neonatal rats were grown on plastic dishes, coated with poly-L-ornithine, for 2-3 days and then were stained with MitoTracker Red CMX-Ros (0.1 µM) on the day of imaging. Fluorescent images were acquired every 2 minutes to visualize mitochondrial distribution and phase images were acquired at 15 to 30 minute intervals to resolve axonal morphology. The time of image acquisition for the phase and fluorescent images is shown as h:min in the movie. The movies were straightened to follow the right axonal branch using the Straighten plugin in ImageJ. A kymograph illustrating the forward advance of mitochondria, stably docked to the axonal framework, is shown on the right hand side of the movie. The kymograph was generated from the straightened image. The length of the arrow above the kymograph represents 1 hour and the vertical scale bar is equal to 20 µm. The dot that moves across the top of the kymograph denotes the time position in the movie. The movie is displayed at 20 frames per second. |
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Source | Lamoureux P, O'Toole M, Heidemann S, Miller K (2010). "Slowing of axonal regeneration is correlated with increased axonal viscosity during aging". BMC Neuroscience. DOI:10.1186/1471-2202-11-140. PMID 20973997. PMC: 2975647. | ||
Author | Lamoureux P, O'Toole M, Heidemann S, Miller K | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 22:31, 20 November 2012 | 4.0 s, 342 × 365 (403 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 1 |
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Author | Lamoureux P, O'Toole M, Heidemann S, Miller K |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010 |