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File:Tlc sequence.png

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Description A mixture is added to the base line (1) shown as purple spots. The TLC plate is palced in a solvent tank and the solvent moves up the plate. A lid ensures that the atmosphere in the tank is saturated with solvent. As the solvent moves up the plate the purple mixture separates into a fast moving red spot and a slower moving blue spot. The Rf of each spot is worked out by measuring the distance the spot moved relative to the distance the solvent moved i.e. for the red spot the Rf is = (1 to 3 / 1 to 4) and for the blue spot the Rf is = (1 to 2 / 1 to 4). The Rf of the red spot is larger, meaning that it has moved further along the plate (in that solvent) than has the blue spot. In this case it can be seen that the purple spot can be separated easily into 2 spots using the light blue solvent.
Date 06/12/09
Source wiki
Author Quantockgoblin

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Public domain I, the copyright holder of this work, release this work into the public domain. This applies worldwide.
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current09:52, 25 September 2008Thumbnail for version as of 09:52, 25 September 2008933 × 128 (5 KB)Quantockgoblin~commonswiki (talk | contribs){{Information |Description= |Source= |Date= |Author= |Permission= |other_versions= }}
22:28, 26 January 2007Thumbnail for version as of 22:28, 26 January 2007800 × 164 (4 KB)Quantockgoblin~commonswiki (talk | contribs){{Information |Description= |Source= |Date= |Author= |Permission= |other_versions= }} Category:chromatography

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