File:Video 5 Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration.ogv
Video_5_Avian_Primordial_Germ_Cells_Contribute_to_and_Interact_With_the_Extracellular_Matrix_During_Early_Migration.ogv (Ogg Theora video file, length 6.0 s, 468 × 468 pixels, 1.37 Mbps, file size: 1,003 KB)
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Summary
edit| Description | Video S5. Cropped area from the lower left region of the movie in Video S2 shown with increased magnification. Live confocal microscope imaging of the lateral germinal crescent region of an HH4 [Tg(hUbC:H2B-Cerulean-2A-Dendra2)] quail embryo that was injected with an antibody against fibronectin (abB3/D6). The antibody was directly conjugated to AlexaFluor 594 (red). The 20x images are maximum intensity projections of 3 optical slices (2.3 μm each) taken from the dorsal aspect at 1.5 min intervals. Total elapsed time is 43 min. Cell nuclei = H2B-Cerulean (blue), cell bodies = Dendra2 (green). PGCs are larger and brighter than the surrounding tissue and appear in clusters and individually. Anterior to top, mediolateral midline to the right out of view. Embryonic tissue to the lower right. Extra-embryonic tissue to the left and upper left. Scale bar = 25 μm. |
| Date | |
| Source | https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2019.00035/full#supplementary-material https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2019.00035/full Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration. Front. Cell Dev. Biol. 7:35. doi: 10.3389/fcell.2019.00035 |
| Author | Huss DJ, Saias S, Hamamah S, Singh JM, Wang J, Dave M, Kim J, Eberwine J and Lansford R |
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© 2019 Huss, Saias, Hamamah, Singh, Wang, Dave, Kim, Eberwine and Lansford. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 20:27, 11 July 2024 | 6.0 s, 468 × 468 (1,003 KB) | Rasbak (talk | contribs) | {{Information |description=Video S5. Cropped area from the lower left region of the movie in Video S2 shown with increased magnification. Live confocal microscope imaging of the lateral germinal crescent region of an HH4 [Tg(hUbC:H2B-Cerulean-2A-Dendra2)] quail embryo that was injected with an antibody against fibronectin (abB3/D6). The antibody was directly conjugated to AlexaFluor 594 (red). The 20x images are maximum intensity projections of 3 optical slices (2.3 μm each) taken from the do... |
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