File:Video 8 Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration.ogv
Video_8_Avian_Primordial_Germ_Cells_Contribute_to_and_Interact_With_the_Extracellular_Matrix_During_Early_Migration.ogv (Ogg Theora video file, length 5.7 s, 512 × 512 pixels, 1.03 Mbps, file size: 715 KB)
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Summary
edit| Description | Video S8. Post-CSAT antibody injection movie for embryo 2. See Video S7 for pre-CSAT injection cell movements. CSAT antibodies against integrin β1 subunits fail to affect the cell motility and morphology of PGCs in HH5 embryos. Time-lapse confocal microscope imaging of the central germinal crescent region of an HH5 [Tg(hUbC:H2B-Cerulean-2A-Dendra2)] quail embryo that was injected with an antibody against fibronectin (abB3/D6). The antibody was directly conjugated to AlexaFluor 594 (red). After incubation, the germinal crescent region was injected again, this time with CSAT antibodies (174 ug/ml) and imaged live. The 20x images, with 2x optical zoom are maximum intensity projections of 3 optical slices (2.3 μm each) taken from the dorsal aspect at 1.5 min intervals. Total elapsed time is 24 min. Cell nuclei = H2B-Cerulean (blue), cell bodies = Dendra2 (green). PGCs are larger and brighter than the surrounding tissue and appear individually and in clumps in this movie. Anterior to the top. Embryonic tissue toward the bottom. Extra-embryonic tissue at the top. Scale bar = 25 μm. |
| Date | |
| Source | https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2019.00035/full#supplementary-material https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2019.00035/full Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration. Front. Cell Dev. Biol. 7:35. doi: 10.3389/fcell.2019.00035 |
| Author | Huss DJ, Saias S, Hamamah S, Singh JM, Wang J, Dave M, Kim J, Eberwine J and Lansford R |
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© 2019 Huss, Saias, Hamamah, Singh, Wang, Dave, Kim, Eberwine and Lansford. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 20:41, 11 July 2024 | 5.7 s, 512 × 512 (715 KB) | Rasbak (talk | contribs) | {{Information |description=Video S8. Post-CSAT antibody injection movie for embryo 2. See Video S7 for pre-CSAT injection cell movements. CSAT antibodies against integrin β1 subunits fail to affect the cell motility and morphology of PGCs in HH5 embryos. Time-lapse confocal microscope imaging of the central germinal crescent region of an HH5 [Tg(hUbC:H2B-Cerulean-2A-Dendra2)] quail embryo that was injected with an antibody against fibronectin (abB3/D6). The antibody was directly conjugated to... |
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