English: (in support of Fig. 7 and Figure S3) Tracing of extracellular MCMV trafficking. M2-10B4 cells were seeded at low density on glass-bottomed culture dishes and infected at a MOI of 100 with gradient purified S-GFP-SCP virus stock. Directly after infection fluorescence live cell imaging with 488 nm laser excitation in parallel with differential interference contrast (DIC) microscopy was started in an environmentally controlled chamber. Virus particles attached to cell protrusions were identified by their fluorescence and recorded with 1.8 frames per second. The video is an overlay of the DIC and GFP channels.