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File:A-Bispecific-Antibody-Promotes-Aggregation-of-Ricin-Toxin-on-Cell-Surfaces-and-Alters-Dynamics-of-pone.0156893.s010.ogv

A-Bispecific-Antibody-Promotes-Aggregation-of-Ricin-Toxin-on-Cell-Surfaces-and-Alters-Dynamics-of-pone.0156893.s010.ogv ‎(Ogg Theora video file, length 23 s, 512 × 512 pixels, 257 kbps, file size: 722 KB)

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English: Live cell imaging of ricin-FITC and late endosomes. Real time imaging of A549 cells visualizing ricin-FITC in green and late endosomes in red labeled with a transient transfection as described in Material and Methods. The total fluorescence for the late endosomes marker is always overwhelming compared to ricin-FITC and therefore the absolute co-localization between ricin-FITC and the late endosomes is not apparent in the raw images prior to processing. Real time images were processed prior to quantification as described in Materials and Methods to determine co-localization values, which are reported in Fig 4C. Total length of the time lapse is 90 min.
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Source S4 Movie from Herrera C, Klokk T, Cole R, Sandvig K, Mantis N (2016). "A Bispecific Antibody Promotes Aggregation of Ricin Toxin on Cell Surfaces and Alters Dynamics of Toxin Internalization and Trafficking". PLOS ONE. DOI:10.1371/journal.pone.0156893. PMID 27300140. PMC: 4907443.
Author Herrera C, Klokk T, Cole R, Sandvig K, Mantis N
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Date/TimeThumbnailDimensionsUserComment
current02:17, 13 October 201623 s, 512 × 512 (722 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 91 kbps Completed 15:44, 14 February 2023 3.0 s
Streaming 480p (VP9) 91 kbps Completed 02:15, 17 December 2023 2.0 s
VP9 360P 51 kbps Completed 15:44, 14 February 2023 3.0 s
Streaming 360p (VP9) 51 kbps Completed 15:38, 17 December 2023 1.0 s
VP9 240P 27 kbps Completed 15:44, 14 February 2023 2.0 s
Streaming 240p (VP9) 27 kbps Completed 02:59, 9 December 2023 1.0 s
WebM 360P 184 kbps Completed 15:44, 14 February 2023 1.0 s
Streaming 144p (MJPEG) 60 kbps Completed 06:37, 29 October 2023 0.0 s

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