English: Time-lapse video microscopy of C. trachomatis inclusion fusion. Video S3 shows the fusion of inclusion harboring GFP- or mCherry-expressing C. trachomatis. HeLa cells were co-infected with a C. trachomatis strain that expressed GFP (green) or mCherry (red) under the control of the incD promoter. 10 h post infection, cells containing several GFP- and mCherry-positive bacteria were monitored by spinning disc confocal microscopy. A z-stack of 56 images covering 14 µm was acquired every 30 minutes. The QuickTime video was generated using Improvision Volocity. The time (hours: minutes: seconds) is indicated in the upper right corner. The video is played at 2 frames/s. Scale Bar: 19 µm.