English: Intraluminal crawling of activated 2D2 GFP CD4+ T cells in inflamed cervical spinal cord post-capillary venules during experimental autoimmune encephalomyelitis (EAE). Active EAE was induced as described in Section “Methods.” Laminectomy was performed (described in step 15 of the procedures) on day 17 post-immunization when the mouse showed the clinical score of 1 (hind leg weakness). In vitro activated CD4+ T cells from “2D2-GFP”-mice were injected via a carotid catheter before 2P-IVM imaging. Contrast enhancement of blood vessels was achieved by injection of Texas Red-dextran (MW = 70,000). GFP (green, CD4+ T cells) and Texas Red were excited at 780 nm. A x–y–t time-lapse sequence of a 150 μm × 150 μm scan field at a depth of 59–76 μm and 11 z-stacks with 1.7 μm spacing is shown. Two CD4+ T cells are seen, which crawl within the post-capillary venule. Time is shown in minutes and seconds. Scale bar: 20 µm. This video is the source video of which images shown in Figure 6 were taken.