File:A-Novel-Family-of-Toxoplasma-IMC-Proteins-Displays-a-Hierarchical-Organization-and-Functions-in-ppat.1001094.s008.ogv
A-Novel-Family-of-Toxoplasma-IMC-Proteins-Displays-a-Hierarchical-Organization-and-Functions-in-ppat.1001094.s008.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 43 s, 320 × 240 pixels, 88 kbps overall, file size: 456 KB)
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editDescriptionA-Novel-Family-of-Toxoplasma-IMC-Proteins-Displays-a-Hierarchical-Organization-and-Functions-in-ppat.1001094.s008.ogv |
English: ISP1 early bud rings in oryzalin treated Toxoplasma. Parasites stably expressing ISP1-HA were allowed to infect HFFs in the presence of 0.5 µM oryzalin and grow 30 hours before fixation and IFA analysis. Serial sections were acquired, deconvolved and projected as a three-dimensional image. In the absence of cortical microtubules, a single mother parasite repeatedly fails to undergo productive replication. Numerous ISP1-labeled rings (red) are found clustered in the center of the cell. IMC1, shown in green, does not co-localize with these structures. Red: anti-HA antibody detected by Alexa594-anti-rabbit IgG. Green: anti-IMC1 antibody detected by Alexa488-anti-mouse IgG. |
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Source | Video S2 from Beck J, Rodriguez-Fernandez I, Cruz de Leon J, Huynh M, Carruthers V, Morrissette N, Bradley P (2010). "A Novel Family of Toxoplasma IMC Proteins Displays a Hierarchical Organization and Functions in Coordinating Parasite Division". PLOS Pathogens. DOI:10.1371/journal.ppat.1001094. PMID 20844581. PMC: 2936552. | ||
Author | Beck J, Rodriguez-Fernandez I, Cruz de Leon J, Huynh M, Carruthers V, Morrissette N, Bradley P | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 13:53, 17 November 2012 | 43 s, 320 × 240 (456 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Beck J, Rodriguez-Fernandez I, Cruz de Leon J, Huynh M, Carruthers V, Morrissette N, Bradley P |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Date and time of digitizing | 2010-09 |
Language | English |