File:A-Role-for-the-Chaperone-Complex-BAG3-HSPB8-in-Actin-Dynamics-Spindle-Orientation-and-Proper-pgen.1005582.s011.ogv

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English: Related to Fig 4E and 4F —Spindle behavior upon introduction of wild type BAG3-GFP in BAG3-depleted cells. Video microscopy of a representative HeLa-RFP-H2B cells expressing low levels RFP-a-tubulin, which have been processed for knockdown-rescue. Mitotic cells expressing comparable levels of Bag3-GFP and variable expression of RFP-H2B were imaged for a 1 h to 1.5 h-time periods at ~1.5 min intervals using a Perkin Elmer UltraVIEW Spinning Disc Confocal and 40x air 0.75NA objective; single plane images are displayed at 7 frames/sec.
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Source S5 Movie from Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, Elowe S, Landry J, Lavoie J (2015). "A Role for the Chaperone Complex BAG3-HSPB8 in Actin Dynamics, Spindle Orientation and Proper Chromosome Segregation during Mitosis". PLOS Genetics. DOI:10.1371/journal.pgen.1005582. PMID 26496431. PMC: 4619738.
Author Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, Elowe S, Landry J, Lavoie J
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Date/TimeThumbnailDimensionsUserComment
current12:45, 8 December 20155.4 s, 704 × 704 (713 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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