File:A-Role-for-the-Chaperone-Complex-BAG3-HSPB8-in-Actin-Dynamics-Spindle-Orientation-and-Proper-pgen.1005582.s021.ogv

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English: Related to Fig 7D —siBAG3-treated cells show spectacular blebbing of the mitotic cortex along with displacement of the mitotic spindle pushed into the bleb. Video microscopy of a representative HeLa-RFP-H2B cell transfected with siBAG3 and transduced with BacMam-GFP-a-tubulin and RFP-actin; cells were imaged for a 1 h-time period at ~1.5 min intervals using a Perkin Elmer UltraVIEW Spinning Disc Confocal and 40x air 0.75NA objective and single plane images are displayed at 7 frames/sec. Please note that RFP-H2B expression is variable in this cell line.
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Source S15 Movie from Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, Elowe S, Landry J, Lavoie J (2015). "A Role for the Chaperone Complex BAG3-HSPB8 in Actin Dynamics, Spindle Orientation and Proper Chromosome Segregation during Mitosis". PLOS Genetics. DOI:10.1371/journal.pgen.1005582. PMID 26496431. PMC: 4619738.
Author Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, Elowe S, Landry J, Lavoie J
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Date/TimeThumbnailDimensionsUserComment
current12:48, 8 December 20154.4 s, 720 × 720 (313 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 720P 247 kbps Completed 22:06, 15 August 2018 2.0 s
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VP9 480P 142 kbps Completed 22:06, 15 August 2018 2.0 s
Streaming 480p (VP9) 143 kbps Completed 03:57, 17 December 2023 1.0 s
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