File:A-microRNA-initiated-DNAzyme-motor-operating-in-living-cells-ncomms14378-s4.ogv
No higher resolution available.
A-microRNA-initiated-DNAzyme-motor-operating-in-living-cells-ncomms14378-s4.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 25 s, 787 × 576 pixels, 201 kbps overall, file size: 625 KB)
File information
Structured data
Captions
Summary
editDescriptionA-microRNA-initiated-DNAzyme-motor-operating-in-living-cells-ncomms14378-s4.ogv |
English: Supplementary Movie 2 Images of MDA-MB-231 cancer cells following intracellular operation of the DNAzyme motor, monitored over 60 min. MDA-MB-231 cancer cells were incubated with the DNAzyme motor for 2 h. The cells were washed and then Mn2+ and the operating buffer were added. Immediately after the addition of Mn2+, fluorescence images of the cells were repeatedly acquired for 60 min at a rate of 2 frames per min. The observed fluorescence in the cells is a result of the intracellular operation of the DNAzyme motor initialed by the target microRNA (miR-10b) in the cells and activated by the cofactor Mn2+. An Olympus IX-81 fluorescence microscope coupled with a Yokagawa CSU × 1 spinning disk confocal scanhead was used. The LMM5 laser transmission setting was 20 and the laser excitation time was 495 ms for each frame of the fluorescence images. |
||
Date | |||
Source | Video file from Peng H, Li X, Zhang H, Le X (2017). "A microRNA-initiated DNAzyme motor operating in living cells". Nature Communications. DOI:10.1038/ncomms14378. PMID 28262725. PMC: 5343503. | ||
Author | Peng H, Li X, Zhang H, Le X | ||
Permission (Reusing this file) |
This file is licensed under the Creative Commons Attribution 4.0 International license.
|
||
Provenance InfoField |
|
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 14:42, 21 May 2017 | 25 s, 787 × 576 (625 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
Transcode status
Update transcode statusMetadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Short title | Supplementary Movie 2 |
---|---|
Author | Peng H, Li X, Zhang H, Le X |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Images of MDA-MB-231 cancer cells following intracellular operation of the DNAzyme motor, monitored over 60 min. MDA-MB-231 cancer cells were incubated with the DNAzyme motor for 2 h. The cells were washed and then Mn2+ and the operating buffer were added. Immediately after the addition of Mn2+, fluorescence images of the cells were repeatedly acquired for 60 min at a rate of 2 frames per min. The observed fluorescence in the cells is a result of the intracellular operation of the DNAzyme motor initialed by the target microRNA (miR-10b) in the cells and activated by the cofactor Mn2+. An Olympus IX-81 fluorescence microscope coupled with a Yokagawa CSU × 1 spinning disk confocal scanhead was used. The LMM5 laser transmission setting was 20 and the laser excitation time was 495 ms for each frame of the fluorescence images. |
Software used | |
Date and time of digitizing | 2017-03-06 |