English: ATX-2 is required for normal cytokinesis. Embryos expressing GFP-MOE marking Actin. Arrows indicate initial position of cytokinetic cleavage furrow. In the control embryo, the cleavage furrow starts to form on both dorsal and ventral (DV) sides of the embryo symmetrically, and extend toward the center of the embryo, then meet at the center to complete cytokinesis. In the atx-2(RNAi) embryo, the cleavage furrow only forms on one side of the embryo, and progresses to the center of the cell but is stalled at two-thirds of DV axis, resulting in cytokinesis failure. In the atx-2(RNAi) embryo, cortical distribution of actin appears disorganized and scattered. Note that in the atx-2(RNAi) embryo, position of the cleavage furrow is not sustained throughout cytokinesis. Movies are Z-projections of the middle 2–3 μm of the embryo. Each time frame is equal to 30 sec of elapsed time. (t = 0 at first indication of cleavage furrow formation; 5 fps). Bar, 5 μm.