File:Activated-T-Cell-Trans-Endothelial-Migration-Relies-on-Myosin-IIA-Contractility-for-Squeezing-the-pone.0075151.s004.ogv
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DescriptionActivated-T-Cell-Trans-Endothelial-Migration-Relies-on-Myosin-IIA-Contractility-for-Squeezing-the-pone.0075151.s004.ogv |
English: Control and MyoIIA KO T cell migration over endothelial cells during TEM. Fluorescently labeled control (green) and MyoIIA KO (red) activated T cells were mixed at a 1:1 ratio and perfused into a flow chamber containing a monolayer of bEnd.3 brain endothelial cells and then kept under physiological shear flow for 15 min. Phase contrast, green and red fluorescence images were acquired every 15 sec. The color-coded tracks show the migration paths of each T cell during the time-lapse. Time in min: sec. |
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Source | Movie S4 from Jacobelli J, Estin Matthews M, Chen S, Krummel M (2013). "Activated T Cell Trans-Endothelial Migration Relies on Myosin-IIA Contractility for Squeezing the Cell Nucleus through Endothelial Cell Barriers". PLOS ONE. DOI:10.1371/journal.pone.0075151. PMID 24069389. PMC: 3777879. | ||
Author | Jacobelli J, Estin Matthews M, Chen S, Krummel M | ||
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current | 14:26, 27 September 2013 | 6.1 s, 1,012 × 756 (1.06 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Jacobelli J, Estin Matthews M, Chen S, Krummel M |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Control and MyoIIA KO T cell migration over endothelial cells during TEM. Fluorescently labeled control (green) and MyoIIA KO (red) activated T cells were mixed at a 1:1 ratio and perfused into a flow chamber containing a monolayer of bEnd.3 brain endothelial cells and then kept under physiological shear flow for 15 min. Phase contrast, green and red fluorescence images were acquired every 15 sec. The color-coded tracks show the migration paths of each T cell during the time-lapse. Time in min: sec. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |