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File:Advances-in-establishment-and-analysis-of-three-dimensional-tumor-spheroid-based-functional-assays-1741-7007-10-29-S6.ogv

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English: Video 3. Tumor spheroid (TS)-embryoid body (EB) confrontation culture assay. 17-N-Allylamino-17-demethoxygeldanamycin (17-AAG) inhibits U-87 MG tissue invasion. Single day 4 green fluorescent protein (GFP) transduced U-87 MG TS and day 7 EBs were cocultured in each well of ultra-low attachment (ULA) 96-well plates. Cocultures were treated with 17-AAG (5 μM) or with vehicle (control) at the initiation of the cocultures. Timelapse imaging shows the invasion of U-87 MG GFP cells towards the EB in the control coculture while no invasion is evident in the 17-AAG treated culture. Images were obtained as described above over a period of 40 h. File format: MPEG.
Date
Source Vinci M, Gowan S, Boxall F, Patterson L, Zimmermann M, Court W, Lomas C, Mendiola M, Hardisson D, Eccles S (2012). "Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation". BMC Biology. DOI:10.1186/1741-7007-10-29. PMID 22439642. PMC: 3349530.
Author Vinci M, Gowan S, Boxall F, Patterson L, Zimmermann M, Court W, Lomas C, Mendiola M, Hardisson D, Eccles S
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current10:54, 20 November 201212 s, 853 × 480 (270 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 117 kbps Completed 21:20, 16 August 2018 7.0 s
Streaming 480p (VP9) 114 kbps Completed 15:03, 17 December 2023 1.0 s
VP9 360P 67 kbps Completed 21:20, 16 August 2018 5.0 s
Streaming 360p (VP9) 64 kbps Completed 21:18, 1 January 2024 1.0 s
VP9 240P 42 kbps Completed 21:20, 16 August 2018 4.0 s
Streaming 240p (VP9) 39 kbps Completed 16:13, 9 December 2023 1.0 s
WebM 360P 263 kbps Completed 15:14, 20 November 2012 7.0 s
Streaming 144p (MJPEG) 549 kbps Completed 20:24, 29 October 2023 1.0 s
Stereo (Opus) 2 kbps Completed 22:55, 14 November 2023 1.0 s
Stereo (MP3) 128 kbps Completed 08:54, 29 October 2023 1.0 s

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