File:Atomic-Force-Microscopy-Protocol-for-Measurement-of-Membrane-Plasticity-and-Extracellular-Video1.ogv
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editDescriptionAtomic-Force-Microscopy-Protocol-for-Measurement-of-Membrane-Plasticity-and-Extracellular-Video1.ogv |
English: Represents contact force mode recording (available in online version). Raw force curves generated using FN-coated AFM probe (also see Figure 2 for details). FN-coated cantilever (1 mg/ml) were controlled to repeatedly approach (black trace) and retract (red trace) from cell while xy-axes fixed (right panel). The snap-off that represents bond rupture, termed adhesion force (left panel raw red traces). The example trace shows 3 adhesion events (bond rupture) between FN ligand (gradient brown circle under gray AFM probe in right panel) and 3-integrin receptors (gradient blue Y-shape) in the cell that occurred when the FN coated-probe retracted. |
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Source | Video 1 from Wu X, Muthuchamy M, Reddy D (2016). "Atomic Force Microscopy Protocol for Measurement of Membrane Plasticity and Extracellular Interactions in Single Neurons in Epilepsy". Frontiers in Aging Neuroscience. DOI:10.3389/fnagi.2016.00088. PMID 27199735. PMC: 4854888. | ||
Author | Wu X, Muthuchamy M, Reddy D | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 01:13, 23 May 2016 | 28 s, 1,440 × 1,080 (1.35 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Wu X, Muthuchamy M, Reddy D |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Represents contact force mode recording (available in online version). Raw force curves generated using FN-coated AFM probe (also see Figure 2 for details). FN-coated cantilever (1 mg/ml) were controlled to repeatedly approach (black trace) and retract (red trace) from cell while xy-axes fixed (right panel). The snap-off that represents bond rupture, termed adhesion force (left panel raw red traces). The example trace shows 3 adhesion events (bond rupture) between FN ligand (gradient brown circle under gray AFM probe in right panel) and 3-integrin receptors (gradient blue Y-shape) in the cell that occurred when the FN coated-probe retracted. |
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Date and time of digitizing | 2016-05-04 |