English: Video of sequential Z-slices of a portion of a cyst taken with FIB-SEM showing the inclusion membrane (putative outermembrane). The broad cell wall of smaller early stage cysts, detected in histology, revealed itself to be an intricate tubular, or vesicular network along the outer side of the inclusion membarne, often enmeshing mitochondria as well as darkly staining deposits, possibly lipid containing, and intimately connecting with the chlamydial inclusion membrane. The amorphous nature of the RBs and the variable number of electron dense nucleoids can readily be seen.